ASUNCION, Paraguay (AP) _ Five opposition politicians were released Friday evening after being kept in custody for 24 hours for holding a political meeting ina private home.

They said they would continue their activism against the 32-year regime of President Gen. Alfredo Stroessner. One of those released, Hermes Saguier, told The Associated Press, ''They'll have to kill us if they want to prevent political meetings.''

Relatives of the five had told the AP earlier that the leaders of the Authentic Radical Liberal Party were among more than 40 people arrested Thursday night during an assembly at a private home in Yaguaron, 28 miles southeast of the capital.

The government does not recognize the party as legal.

All but the five, who were transferred to police headquarters in the capital, were immediately released after being questioned by police.

A police spokesman said the politicians were detained because they did not have permission from the Interior Ministry to meet.

In addition to Saguier, they were Nelson Garcia, Juan Masi, Francisco de Vargas and Elba Recalde.